The Genomics group provides comprehensive and integrated genomics analysis for the Unit Programmes and external collaborative projects.
The group carries out a range of genomic analyses, and is fully equipped to extract, purify, quantify and QC RNA and DNA for further investigation. Currently the facility has the following sequencers and array technology systems:
1) ThermoFisher Scientific (formerly LifeTechnology) Ion Torrent PGM™
2) Illumina MiSeq Sequencer
3) Agilent Microarray technologies
4) ThermoFisher Scientific QuantStudio™ 6 Flex Real-Time PCR System (96- and 384-well capability)
The group provides support, training and analysis for a range of applications, including gene expression analysis, miRNA analysis, qPCR, ChiP-Seq, small genome and targeted genome sequencing (off-the-shelf and custom panels), polysome profiling and ribosome profiling.
Bioinformatic support for microarrays is provided using GeneSpring software (Agilent technologies).
For more advanced informatics of sequencing data the Genomics group works closely with members of the Bioinformatics group.
In addition to our in-house sequencers, the Genomics group also prepare libraries (multiplexed if necessary) for ‘deep-sequencing’, through out-sourcing links with TGAC (The Genome Analysis Centre) in Norwich and the department of Biochemistry at the University of Cambridge. This provides access to ‘state-of-the-art’ Illumina HiSeq and NextSeq next generation Sequencers and allows any scale of project to be handled.
We have developed a pipeline for performing ribosome profiling.
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