Next Generation Sequencing Facilities
We use the Ion Torrent PGM and Illumina MiSeq bench-top Sequencers.
We have the capability to perform small genome and targeted genome sequencing using AmpliSeq and RNA-Seq pipelines. We have used both off-the-shelf and custom-made panels (e.g. the Cancer HotSpot panel and the Lung and Colon panel) and have generated successful libraries from difficult to work with samples, such as circulating free DNA and FFPE tissue.
We advise collaborators on planned projects and offer full training if required.
For larger scale projects, requiring whole genome sequencing, or ribosome profiling experiments, we offer a library preparation service. We generate libraries, QC and pool them, and then co-ordinate out-sourcing to the relevant facilities.