Cryo-Electron Microscopy of Vitreous Sections
1) Vitrificate tissues & cells by HPF or slam freezing.
2) Trim the frozen samples.
3) Ultra cut the ribbon-like sections at liquid nitrogen temperature.
4) Pick up the cryo-sections on grid.
5) Transfer the cryo-grids to the sample holder.
6) General alignments.
7) Follow same procedure as for TEM Tomography.