CEMOVIS

Cryo-Electron Microscopy of Vitreous Sections

Leica EM HPM100, Variant Instruments, Leica EM UC7/FC7, Leica Crion/Manipulator

Workflow:

1) Vitrificate tissues & cells by HPF or slam freezing.

2) Trim the frozen samples.

3) Ultra cut the ribbon-like sections at liquid nitrogen temperature.

4) Pick up the cryo-sections on grid.

5) Transfer the cryo-grids to the sample holder.

6) General alignments.

7) Follow same procedure as for TEM Tomography.

Latest News