CLEM

We use Correlation Light and Electron Microscopy (CLEM).

Gridded coverslips, Leica 3mm/6mm CLEM system

Items required:

1) Photoetch gridded coverslip for chemical fix.

2) Gridded glass-bottom dish by photo-etch for chemical fix.

3) Gridded sapphire coverslip by carbon-coat for HPF.

 

 

Workflow:

1) Culture tissue cells on the gridded coverslips.

2) Observe cells using FM or phase contrast LM with gridded patterns.

3) Chemically fix with glutar/paraformaldehyde (or vitrificate by high-pressure freezing).

4) Plastic embedding.

5) Trim the gridded pattern on the plastic.

6) Ultra-section.

7) Conventional 2D-TEM (or Gatan 3View system).

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