Negative Staining

We use negative staining to visualise purified protein-complexes.

JEOL JEM 100CX TEM with Olympus MegaView. FEI Talos TEM with CETA.

Workflow:

1) Glow-discharge the carbon coated Formvar grid.

2) Put purified protein-complexes on the grid.

3) Stain the grid with uranyl acetate (fixed with glutar if necessary).

4) Observe specimen using MegaView prior to single particle analysis.

5) Observe specimen using CETA16 for 2D classification.

 

 

Negatively stained images of purified protein-complexes.

Stained with uranyl acetate.

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