Single Particle Analysis with Cryo EM

We use single particle analysis for purified protein-complexes.

TEI Talos FE-TEM with FEI Ceta/Falcon CMOS Detector

Workflow:

1) Wash Quantifoil grids with acetone.

2) Vitrificate purified protein-complexes on the grid by FEI Vitrobot.

3) Set the cryo grids on the sample holder.

4) General alignments.

5) Start the FEI EPU (Atlas acquisition/square selection/ice filter location selection/template definition/automated acquisition for automatic image acquisition on Ceta16 & Falcon2 direct electron detectors.

6) Image processing by EMAN & RELION (particle classification/align & average classes/3D model/projections/repeat procedures).

 

 

EPU images of purified protein-complexes by motion correction on Falcon

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