Dr. Claudia Langlais

Higher Scientific Officer - Protein purification/subcellular fractionation/mass spectrometry

Headshot of Dr Claudia Langlais

MRC Toxicology Unit
Hodgkin Building
PO Box 138
University of Leicester
Lancaster Road, Leicester

Email: cl40@le.ac.uk

Qualifications and Personal History

Over 14 years’ experience in a range of biochemistry techniques including SDS-PAGE, Western Blotting, silver and coomassie staining, as well as protein purification methodologies such as gel filtration, ion exchange and affinity chromatography using AKTA systems (SMART System, AKTAprime, AKTApurifier, AKTAmicro). Experience in subcellular fractionation, immunoprecipitation techniques in addition to mammalian and insect cell culture. Around 6 years’ experience in mass spectrometry (Synapt G2Si and Q-TOF2) including sample preparation, data acquisition, troubleshooting, and analysis of mass spectrometry data using various software packages (PLGS, Scaffold, ProgenesisQI, ISOQuant).

2005-Present Research Scientist, Protein Profiling Group, MRC Toxicology Unit, UK.

2002-2004 Post-Doc, RZPD (Resource Centre for Genome Research), Heidelberg, Germany.

2002 PhD: “Characterisation of the ~700kDa Apoptosome Complex from THP.1 Cells”, University of Leicester, UK.

1998 MSc, Toxicology: “In Vitro metabolism of the phytoestrogen Genistein”, University of Surrey, UK.

1996 BSc, Pharmacy, Albert-Ludwigs University, Freiburg i. Br., Germany.

Research Interests

Key area of scientific interest is mitochondrial proteomics. I recently established a robust novel method for isolation of mitochondria from cultured cells and leukaemic patient samples for analysis by mass spectrometry. I have then carried out detailed analysis of the leukaemic mitochondrial proteome (submitted for publication). I have also collaborated with G.Miles to determine how metabolic programming affects the mitochondrial proteome and apoptotic cell death. My experience in protein purification techniques, in particular gel filtration, has aided the protein profiling group in implementing improved methodologies for sample purification prior to mass spectrometry analysis and I have set up a suite of three AKTA systems for this purpose. I have also become an expert in using the Progenesis QI software for analysing and quantifying protein expression in cells, complexes and mitochondria. Collaborations within the Toxicology Unit include providing guidance in the planning and execution of experiments for mass spectrometry, performing MS analyses (sample preparation, data acquisition and data analysis), as well as assisting groups with protein purification projects and immunoprecipitation methodologies.

Selected Publications

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