Higher Scientific Officer - Mass Spectrometry Specialist
MRC Toxicology Unit
PO Box 138
University of Leicester
Lancaster Road, Leicester
LE1 9HN, UK
Tel: +44 (0)116 252 5573
Qualifications and Personal History
BSc Hons, Biological Chemistry.
I started work at the MRC Toxicology Unit at Carshalton Beeches in 1985 as a Scientific Officer immediately after completion of a BSc. Hons in Biological Chemistry. I was involved in the study of toxic plant compounds, pyrrolizidine alkaloids, under Dr. Robin Mattocks. After the Unit relocated to Leicester I worked as a synthetic chemist in the Biomarkers Group for Prof. Peter Farmer, where I synthesized standards for use in the elucidation and analysis of novel biomarkers of toxic injury by mass spectrometry.
For the last 13 years I have worked for Prof. Kelvin Cain in the Protein Profiling Group, first using Waters M@ldi and QTof II instruments, latterly, for the last 4 years, using our Synapt G2Si instruments. I have concentrated on establishing robust analytic pipelines for label-free mass spectrometry. This approach is rapidly becoming the method of choice for characterising changes in protein expression, and I have focused on using various software packages to quantify proteins in cells, sub-cellular organelles and multi-protein complexes. The research projects in which the group is involved are many and various, with proteomics projects ranging from characterising proteins involved in apoptotic cell death, DNA repair and protein translation and transcription.
Many of the projects centre on the identification of protein interaction partners and the use of label-free quantification to elucidate disparities between cell types or between treatments. In-gel, in-solution or off-bead digestions are selected depending on the requirements of the experiments and the complexity and amounts of available protein. The samples generated can be analysed by a variety of methods as detailed in the Mass Spectrometry section of these webpages. We advise collaborators on the best methods for achieving their goals and are interactively involved in the design and planning of experiments. In our experience, the best proteomic results are obtained by detailed forward planning of the proteomic experiment and, in this respect, the experience we have gained with other projects is an invaluable aid to the experimental design.
Hughes MA, Powley IR, Jukes-Jones R, Horn S, Feoktistova M, Fairall L, Schwabe JW, Leverkus M, Cain K, MacFarlane M.
Mol Cell. 2016 Mar 17;61(6):834-49. doi: 10.1016/j.molcel.2016.02.023.
Craxton A, Somers J, Munnur D, Jukes-Jones R, Cain K, Malewicz M.
Cell Death Differ. 2015 Jun;22(6):890-7. doi: 10.1038/cdd.2015.22.
King HA, Cobbold LC, Pichon X, Pöyry T, Wilson LA, Booden H, Jukes-Jones R, Cain K, Lilley KS, Bushell M, Willis AE.
Cell Death Differ. 2014 Jan;21(1):161-71. doi: 10.1038/cdd.2013.135.
Althubiti M, Lezina L, Carrera S, Jukes-Jones R, Giblett SM, Antonov A, Barlev N, Saldanha GS, Pritchard CA, Cain K, Macip S.
Cell Death Dis. 2014 Nov 20;5:e1528. doi: 10.1038/cddis.2014.489.
Dickens LS, Boyd RS, Jukes-Jones R, Hughes MA, Robinson GL, Fairall L, Schwabe JW, Cain K, Macfarlane M.
Mol Cell. 2012 Jul 27;47(2):291-305. doi: 10.1016/j.molcel.2012.05.004.
Twiddy D, Brown DG, Adrain C, Jukes R, Martin SJ, Cohen GM, MacFarlane M, Cain K.
J Biol Chem. 2004 May 7;279(19):19665-82.
Chernova T, Steinert JR, Richards P, Mistry R, Challiss RA, Jukes-Jones R, Cain K, Smith AG, Forsythe ID.
Mol Pharmacol. 2011 May;79(5):844-54. doi: 10.1124/mol.110.069831.